Sentence examples for min before applying from inspiring English sources

The plates were dried at ambient temperature for 20 min before applying the E-test strips.

The PPA cell was then inserted in the heating jacket and left there for 30 min before applying pressure, to allow the heat to be transferred to the cell to simulate downhole conditions.

For βGA experiments, cells were pretreated for 20 min before applying H2O2 or CSE.

After each episode, the neuron was returned and maintained at −50 mV for 2 min before applying the subsequent voltage step; the voltage-dependent [Cl−]i shifts, which obligatorily accompany membrane potential migrations [16], were thus minimized.

RNA samples were treated with Turbo DNase (Ambion) at 37°C for 30 min before applying RT-PCR protocols.

Alternatively, devices were sonicated with deionized water or culture media for 5 10 min before applying to Petri dishes.

The mixture was pre-annealed for 30 min at 37°C before applying it to the slides.

Toxin neutralization by the scFv preparations was assessed by mixing 20 LD50.mL−1 (87 pg.mL−1) of BoNT/A1 holotoxin or 10 LD50.mL−1 of BoNT/A2 in complex form with concentrations of the scFv preparations ranging from 0.1 to 27 μg.mL−1 and by incubating for 30 min at 37 °C before applying the mixture to the tissue.

× 20 mm column at 20 μL/min in 99.9% A. The analytical column was then switched in-line and the mobile phase was held for 5 min at 5% B before applying a linear elution gradient of 5% B to 40% B over 90 min at 300 nL/min.

Five μL injections of each sample were trapped for 5 min on a 5 μm Symmetry C18 180 μm I.D. X 20 mm column at 20 μl/min in 99.9% A. The analytical column was then switched in-line and the mobile phase was held for 5 min at 5% B before applying a linear elution gradient of 5% B to 40% B over 90 min at 300 nL/min.

After the sample loading, the C18 column was washed for 35 min with solvent A, before applying the LC gradient.