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The training phase task started 5 min before injection of tracer and ended 5 min before completion of RAC PET (total duration 60 min).
The task started 5 min before injection of tracer and ended 5 min before completion of RAC PET (total duration 60 min).
To determine the dye localization, mitochondria were labeled with NAO (100 nmol/L final concentration) for 20 min before completion of the incubation with IC7-1 derivative.
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Finally, the reaction mixture was maintained at 72 °C for 5 mins before completion.
Samples were obtained at baseline, at 30 and 45 min, just before completion of infusion and hourly during 8 h.
Fentanyl 100 μg was administered 10 min before surgery completion, and continuous infusion of propofol, remifentanil, and phenylephrine was discontinued at the completion of surgery.
Upon completion of this sprint, subjects rested for 5 min before completing the 24 × 6-s test, which consisted of 24 6-s all-out sprints departing every 30 s.
At the final step, the reaction mixture was incubated at 72°C for 5 min before the completion.
One patient with 3 years of hemodialysis requirement experienced significant muscle cramps, requiring hemodialysis to be discontinued 30 min before desired completion (targeted hemodialysis time 240 min; actual was 180 min).
Finally, the reaction mixture was maintained at 72°C for 5 min of primer extension before completion.
Apparently, in most cases, the plasmon intensity at the reaction time of 15 min is close to that at 30 min, meaning completion of reaction on or before 30 min.
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CEO of Professional Science Editing for Scientists @ prosciediting.com