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Three microscope fields were selected at random and photographed.
Fifty microscope fields were examined for the presence of motile parasites at 400× magnification.
At least 4 random microscope fields were analyzed for each sample well and experiments were repeated at least 3 times.
Six random high-power (100x) microscope fields were examined and the average of these six fields was taken.
An average of 6 15 adjacent microscope fields were screened with a total of 50 consecutive tumour cell nuclei measured in each sample.
Colour images from the same 16 adjacent microscope fields were automatically acquired and digitally combined under four different staining conditions, using a Prior computer interfaced stage and Prior controller to revisit the same stage co-ordinates.
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To evaluate the intensity BDNF immunolabeling per medium spiny neuron, the intensity of fluorescent BDNF immunolabeling in each of three 1.0-mm-square confocal microscope fields was first determined in each of three rostrocaudally spaced sections on each hemisphere of 6 mice from each saline, TP10 treated R6/2 mice and wild type littermates.
The number of adherent bacteria in 20 randomly chosen microscope fields was determined using Image J software (version 1.41).
The total length of the tube-like structures in five randomly chosen microscope fields was measured by ImageJ (Bethesda, MD, USA) software.
For quantification of apoptotic cells, a minimum of 100 cells, each from at least three microscope fields, was examined for TUNEL-positive nuclei among the DRAQ5-stained ones.
Briefly, two images of the same microscope field were collected using phase contrast and fluorescence microscopy.
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