For each section, ten randomly selected separate nonoverlapping microscopic fields were examined under light microscope at 400x magnification.
Morphological analyses were performed on Nikon Eclipse Ni microscope (Nikon Co., Tokyo, Japan), and the microscopic fields were digitized.
To determine their length and width, stomata on 25 randomly chosen microscopic fields were counted for each leaf.
Four randomly chosen microscopic fields were captured.
For every experimental series 10 microscopic fields were photographed.
For determining the % co-localization, 50 microscopic fields were counted.
Y. pestis cells in at least 20 random non-overlapping microscopic fields were counted.
The same microscopic fields were imaged before and after treatment for contraction analysis.
Video recordings of microscopic fields were made continuously (1 image/min) using an in house developed software program version of the IPlab 3.5.5.
The microscopic fields were chosen at random and at least 2 3 fields with about 8 10 cells in each field were selected for further analysis.
To quantify alterations in Mitotracker fluorescence, 100 microscopic fields were averaged for their fluorescent intensity (Fuji Image Systems, ImageGauge v. 3.5) and averaged between control and CMP conditions.
