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With a microscope, rings were counted in two directions until the same count was obtained.
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Then the rings were photographed under an inverted microscope, and the number of sprouts per ring was quantified.
The newly invaded rings were counted on giemsa stained smears under a light microscope.
The rings were incubated for 5 days at 37°C and observed using a CK2 Olympus microscope (×100 magnification).
Thereafter, the rings were carefully dismounted from the chambers and PVAT was carefully removed with micro scissors under a stereoscopic zoom microscope.
Aortic rings were photographed on day 10 using a Canon Power Shot S620 digital camera connected to an Axiovert 40c Zeiss microscope and evaluated using ImageJ.
Consequently, growth rings are generally difficult to distinguish even under a microscope.
Rings are old school".
Rings are a must.
Key rings are inexpensive.
Rings are good.
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