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Total 5 random 4×10 magnification fields were selected and average number of colonies of each sample was calculated.
The observations coming from three magnification fields were averaged.
Four randomly selected × 200 magnification fields were photographed, and the number of invading cells was counted.
Three high magnification fields were counted for each tumour section and the mean microvessel density value was recorded for each.
Ten high magnification fields were observed for each slice and 100 cancer cells were counted in each field.
Five high-power (200x magnification) fields were chosen randomly for each section, and 500 cells were counted per field.
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The number of PECAM-1-positive vascular structures in 40× magnification fields was scored along the border of the ventral disc margin.
The number of Tbr2+CR+ cells (arrows) and Tbr2+CR− cells (arrowheads) per high magnification field were quantified in (B′, B′′ ).
Positive cells stained red in high-magnification fields were counted [ 12].
Several nonoverlapping low-magnification fields were photographed on a NIKON fluorescence microscope, and cells were semiautomatically counted using the object count function of NIKON NIS elements.
The number of Tbr2+NeuN+ cells and Tbr2+NeuN− cells per high magnification field was quantified in (B′ ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com