Routine microscope slides were fixed at 3 × 1 inches during the Victorian era and are still produced at the metric equivalent of those dimensions (7.5 × 2.5 cm) today.
Glass microscope slides were cut into pieces with surface areas of 24 mm24
Under the optical microscope, the films on the glass microscope slides were clear and featureless.
For a fungal culture, FISH preparations were conducted for more than ten times, from each of which eight microscope slides were resulted.
Microscope slides were rehydrated in phosphate buffer (PBS) and subsequently blocked with phosphate buffer containing 1% bovine serum albumin (PBS-1%BSA).
After hybridization, the microscope slides were washed at 48 °C for 12 min by immersing them into 50 ml of washing solution.
For the biofilms in the calibration set, ordinary glass microscope slides were used since each biofilm was ultimately transferred from the substrate before Raman analysis, much like a plaque scraping might be transferred.
For the hydrated biofilms of interest in these studies (examined in situ), quartz microscope slides were chosen in order to avoid the high levels of fluorescence associated with using NIR illumination on a glass substrate.
The prepared microscope slides were analysed in 12 transects, each 4 mm apart, to identify pollen and tephra grains and to ascertain the approximate time of the capture of various particles by the trap.
Microscope slides were coated with soot using a candle flame.
Three microscope slides were made from each sample of microresidues from the 48 coprolites.
