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Confocal microscope images were taken to evaluate the cell imaging applications of the fluorescent NDs.
Microscope images were subsequently obtained followed by PA imaging.
Optical microscope images were collected on a Nikon SM2800 microscope.
Microscope images were viewed and recorded using GXCapture version 7.3 software (Suffolk, UK).
Transmission electron microscope images were taken by JEOL JEM-1400 electron microscope.
The optical microscope images were taken in order to identify graphene [15].
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Light-microscope images were photographed, and the total pyramidal cell numbers per millimeter in the hippocampus and in the subregions CA3 were measured on the photographs and then averaged to give a single value.
Depending on treatment, 9 11 brain regions and 4 6 sections for each region were examined using light microscopy (Nikon Eclipse E800 series microscope. Images were captured using a CoolSnapHQ camera system and Metamorph software (using 10Xobjective).
Slides were mounted with 0.1% (v/v) Mowiol (Calbiochem, San Diego, CA, USA) and viewed with a fluorescence microscope [images were recorded with a Spot Insight digital camera (Delta Sistemi) equipped with a system of image analysis (IAS 2000; Delta Sistemi)].
Microscope images were also collected directly from the antifungal assays with an Olympus IX70 inverted microscope.
Microscope images were carried out with a CoolSnap digital camera and Image J software was used for image analysis.
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