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To identify the specific RNAs that were associated with each candidate RBP, the Log2 ratios from each microarray were normalized to the average of the Mock arrays (the average of the Mock arrays was calculated after median normalization).
Raw data derived by oligo microarray were normalized using lowess algorithm exploiting MIDAW web tool [ 19].
Signal intensities for an entire microarray were normalized to the 50% percentile median value.
The Cy3/Cy5 ratios of all spots on the DNA microarray were normalized using the global ratio median.
The Cy3/Cy5 ratios of all spots on the DNA microarray were normalized by the global ratio median.
Log-ratios for each microarray were normalized by the locally robust lowess smoother (20) to adjust for any dye bias and variation in PMT voltage settings.
Similar(52)
MiRNA microarray was normalized by Lowess normalization algorithm using GeneSpring 10.0 (Agilent Technologies, Foster City, CA, USA).
Any bias in signal intensity between the two fluorescent dye channels in a microarray was normalized by locally weighted linear regression analysis or Lowess normalization [16] using MIDAS (freely available from http://www.tm4.org/midas.html).html
The median value of each microarray was normalized to 500.
The Cy-5 test signal was divided by the control signal (genomic pool) and each microarray was normalized to the mean of the control group.
In the final step of pre-processing, each individual experiment (microarray) was normalized by setting the mean to zero and its standard deviation to one, and each gene was median centered.
Related(20)
microarray were examined
microarray were employed
microarray were verified
microarray were analyzed
microarray were investigated
microarray were isolated
microarray was normalized
microarray were obtained
microarray were washed
microarray were quantified
microarray were selected
microarray were taken
microarray were done
microarray were found
microarray were validated
microarray were considered
microarray were plotted
microarray were used
microarray were described
microarray were based
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