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Expression changes seen by microarray were verified by quantitative PCR.
Gene expressions observed in microarray were verified for five selected genes from five samples by quantitative real-time PCR.
In the development of doxorubicin resistance, cell-fusion associated genes, from the previous results of microarray, were verified using dot blot array and quantitative RT-PCR.
All positive samples detected by MassTag PCR, 16S rRNA PCR, and microarray were verified by targeted singleplex PCR amplification and sequencing.
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The efficacy of the DNA microarray was verified in a parallel study where pathogen genes and E. coli cells were enumerated using real-time quantitative PCR (qPCR) and standard membrane filter techniques, respectively.
The expression level of these three miRNAs detected by microarray was verified by qRT-PCR.
The expression level of the three lncRNAs measured by microarray was verified by qRT-PCR (see online supplementary results and supplementary figure S2).
The pattern of gene expression from the microarray was verified by examining the relative transcript levels for candidate Cbf genes normalized to the 18S ribosomal transcript levels by real time qRT-PCR.
Changes in expression of genes of interest from the microarrays were verified using quantitative RT-PCR.
Hybridization quality of the microarrays was verified by scaling factor, overall hybridization rate, and signal strength of several bacterial spike controls.
Microarray results were verified by qPCR.
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