All microarrays were examined for surface defects, grid placement, background intensity, housekeeping gene expression, and a 3'∶5' ratio of probe sets from genes of various lengths.
All of the microarrays were examined for surface defects, grid placement, background intensity, housekeeping gene expression (GAPDH and β-Actin), and 3'-/5'- ratio of probe sets.
All microarrays were examined for surface defects, grid placement, background intensity, housekeeping gene expression, and a 3':5' ratio of probe sets from genes of various lengths.
Tumor microarrays were examined by light microscopy and cores were categorized into either positive or negative staining for TACC3 and TACC1, with staining representing those cores where greater than 10% of the cells had detectable staining, otherwise they were scored.
Having sorted the cells, a global gene expression in cancer cells using DNA microarrays was examined.
Given the sense-antisense architecture of the microarray (see above), all differentially expressed microarray targets were examined to identify and eliminate microarray targets for which both the sense and antisense probes were differentially expressed.
To identify putative candidate genes involved in the regulation of wood stiffness and microfibril orientation, differentially accumulated transcripts identified in a single microarray experiment were examined in the other two microarray experiments.
Biopsies from tumour and control adjacent to the biopsies for microarray analysis, were examined histologically by H. Sandeck to identify which cell types were included in each specimen and also estimate the relative content of cells of each type (per cent of total cell nuclei).
