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Two species highly expressed at 3H by the microarray were selected for western blot analysis.
A total of 27 genes with different levels of expression detected using microarray were selected for real-time PCR verification using the same RNA purified for microarray hybridizations.
Candidates identified from the comparison of follicular tissues from the microarray were selected using a multi-level filtering system.
To verify the results of the microarray analyses, 12 differentially expressed genes based on microarray were selected for qRT-PCR to further investigate the expression profiles.
Sequences targeted by the microarray were selected for quantitative PCR analysis to corroborate the results obtained in the microarray (Table 3).
Six different genes found to be regulated in LsRXR RNAi by the microarray were selected and quantified by real time PCR.
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miR-454, which was the control used in the microarray, was selected as the endogenous control in the plasma, whereas RNU6B was used as the endogenous control in the NPC cell lines and tissues.
Orthologous genes between mouse and human microarrays were selected using HomoloGene database of National Center for Biotechnology Information (NCBI).
Only probe sets that had at least an intensity of 20 and a present call at one of the microarrays were selected.
Breast cancer tissue microarrays were selected based on the availability of paraffin blocks and prepared by extracting two 1-mm diameter cores of histological confirmed invasive breast carcinoma and immunohistochemically stained for ER, PR, basal cytokeratins HER2, CK5/6 and vimentin.
Then, the 15 candidate miRNAs discovered via microarrays were selected for further testing by qRT-PCR in the training phase.
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