Hybridized cDNA microarray were washed, dried, and scanned using Scanarray 4000 (GSI Lumonics, Billerica, MA, USA).
Hybridized slides (Human 4x44K Microarray) were washed as recommended and scanned using the High-Resolution Microarry Scanner (Agilent, Santa Clara, CA, USA).
A double-stranded microarray was washed with PBS 0.01% (v/v) Triton X-100 and blocked with PBS-2PBS-2%v) BSigmaigma) for 1 h.
Subsequently, the microarray was washed with PBS-50 μM zinc acetate-0.5acetate-0.5%n-20 for 10 min, PBS-50 μM zinc aceTween-2010 Triton X-100 for 2 min and PBS-50 μM zinc acetate for 2 min.
The hybridized microarray was washed and then scanned in Cy3 channel with the Agilent DNA Microarray Scanner (model G2565AA).
After hybridization, the microarray was washed 3 times in wash buffer and then labeled for 10 min at 50°C with a solution containing streptavidin-allophycocyanin (1mg/ml final concentration), 6× SSPE, 1× Denhardt's solution, and 0.01% Tween-20.
The microarray was washed twice and fluorescence detected using Agilent's Microarray Scanner System.
Following hybridization, the microarray was washed using the NimbleGen Wash Buffer Kit.
After hybridization, each microarray was washed, then scanned using GenePix 4000A microarray scanner (Axon Instruments, Union City, CA).
After hybridization, the microarray was washed and stained with streptavidin R-phycoerythrin conjugate using an Affymetrix Fluidics Station.
After incubation for 1 hr at room temperature, the microarray was washed in PBST (PBS with 0.5% Tween-20).
