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a Unigenes targeted in microarray were based on contigs assembled from ESTs generated in [ 40].
Primers for qPCR (Additional file 5) and oligonucleotide probes on the microarray were based on the same cDNA sequences.
The probes on the microarray were based on the 42,440 unigene sequences obtained from a 454 RNA-seq dataset in our laboratory [ 24].
Similar(57)
This liquid microarray is based on the 5'UTR — the most highly conserved region of HCV — and the variable region NS5B sequence.
First, our DNA microarray was based on the genome sequence of strains NCTC 11168 and RM1221, and genes that are not present in these strains used to construct the microarray will not be detected.
Additionally, miRNAs detected by microarray are based on reference sequences, however, the reference sequences are often not the dominant ones for some miRNAs, which might be another reason causing that discrepancy.
The mouse CpG island microarray was based on previously described human CpG island microarrays [ 43– 43].
On the other hand, microarray is based on the hybridization of specimen target strands onto the immobilized complementary probe strands.
The principle of microarray is based on the complementary hybridization between nucleotide chains such as DNA-to-RNA strands and DNA-to-DNA strands [ 17].
Because microarray is based on probe-target hybridization, cross hybridization among probes from members of gene families could produce false-positive signals under some hybridization conditions.
The microarray is based on differential hybridization of species-specific oligos between N. vitripennis and Nasonia giraulti at more than 20,000 markers spanning the Nasonia genome.
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