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A flow cell (56×44×1 cm) was filled with glass beads and operated under a continuous flow of a mineral medium containing nitrate as electron acceptor.

Anabaena cells harboring a PhetR::gfp reporter gene were grown in BG-11 liquid medium containing nitrate with aeration with air (normal CO2), followed by four washes with BG-110 media lacking combined nitrogen.

nit-6 encodes the structural gene for nitrite reductase and is only expressed when N. crassa is cultured on medium containing nitrate and lacking a good nitrogen source (typically glutamine or ammonium) (Exley et al. 1993).

These two modes of regulation result in repression of nit-6 during growth on glutamine or ammonium, but result in expression at high levels in medium containing nitrate as the sole nitrogen source (Exley et al. 1993).

A sample of uninduced algae was taken as a reference (as described above) and the remaining culture was washed (5 h before onset of the dark phase in the cycle of 8 h dark and 16 h light) and transferred to Volvox medium containing nitrate instead of ammonium chloride.

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P. purpurogenum IAM15392 has been found to produce amino derivatives of Monascus pigment homolog, PP-V, in culture with a medium containing ammonium nitrate (Ogihara et al. 2000).

Sexual development was initiated by growth on solid minimal medium containing sodium nitrate, proline, or alanine as a nitrogen source.

Using this model, we first identified essential genes and reactions in fermentation medium containing sodium nitrate as a nitrogen source [ 23].

Cultures were grown semi-continuously; after each daily sampling, N-replete and N-deplete cultures were diluted to 10 cells mL−1 with BG-11 medium containing 0.1 M nitrate or no N, respectively, causing increasing N starvation in N-deplete cultures.

Pairing tests were performed in 96 cell plates by growing different mutants of two isolates at 25°C for 2 weeks in a minimal medium containing sodium nitrate as the sole nitrogen source.

He said security officials have temporarily seized 64,000 tonnes of fertilisers containing nitrate from retailers.

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