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These receptors were activated in a medium containing extracellular calcium.
Efficient retinal differentiation occurs when SFEBq aggregates of mouse ESCs are cultured under low growth-factor conditions in a medium containing extracellular matrix proteins (matrigel) [ 28].
Briefly, conditioned Opti-MEM medium containing extracellular proteins was dialyzed against phosphate buffered saline, biotinylated, dialyzed again, hybridized to the array, washed, labeled with streptavidin-Cy3, washed, dried and the fluorescent features imaged on a Molecular Devices 4000b scanner.
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Finally, upon incubation in medium containing this extracellular pool of survivin, cancer cells responded physiologically, becoming more resistant to therapy, proliferating more rapidly, and having an increased metastatic potential.
Cover slips were placed into a perfusion chamber (volume = 500 µl) containing extracellular medium containing (in mM): 144 NaCl, 2.5 KCl, 2.5 CaCl2.52.5 MgCl2, 10 Glucose, 10 Hepes and pH 7.4.
Extracellular stimulation of neurones was performed with a patch electrode containing extracellular medium.
In low iron medium, the Rv3371 deletion mutant showed reduced formation of TAG containing extracellular vesicles.
Stimulation of OSNs with forskolin (20 µM) in extracellular medium containing low Ca2+ (1 nM) failed to generate any detectable NO-release (n = 17 cells), arguing for dependency on extracellular Ca2+.
Hemichannel activity under oxidative stress (induced by CSE and H2O2) was examined by LY uptake from the extracellular medium containing normal Ca++.
After removal of gentamicin IPEC-J2 cells with intracellular Salmonella were incubated with E. coli for 2 hours and then incubated for up to 20 hours with medium containing gentamicin to kill extracellular bacteria.
In this experiment, 4 ml of extracellular medium (containing 5% FBS) was deproteinised, concentrated and analysed by HPLC.
More suggestions(12)
medium containing appropriate
medium containing pyruvate
medium containing exogenous
medium containing high
medium containing free
medium containing antibiotic
medium containing low
medium containing additional
medium containing neomycin
medium containing normal
medium containing gentamicin
medium containing serial
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