Sentence examples for medium containing individual from inspiring English sources

These inoculums were further subcultured to fresh MEB medium containing individual enzyme specific substrates and incubated on shaker at 30°C, 150 rpm for 4 days.

Fresh medium containing individual retinoids was provided every 24 hours.

Tolerance to HMF, furfural and acetate was performed in 1 ml liquid synthetic medium containing the individual inhibitors in a 24-well plate.

First, strains containing individual geneXΔ:: kanMX4 deletions were grown in liquid YES medium in microtiter dishes.

After transduction of E. coli EPI100 cells by the recombinant fosmid library and growth at 37°C on solid LB medium containing 12.5 μg/mL chloramphenicol, individual colonies were transferred to 384-well microtiter plates containing freezing medium (Luria-Bertani, 8% glycerol complemented with 12.5 g/mL chloramphenicol), using an automated colony picker (QPixII, Genetix, UK).

Infected cells were cultured in methylcellulose medium containing IL-7 (Stem Cell Technologies) and individual colonies were isolated on day 7 and transferred into complete IMDM media with 20% fetal bovine serum, but no IL-7.

To study the regeneration ability of protoplasts, the formed individual protoplasts were spread on medium containing NaNO3 0.3%, K2HPO4 0.1%, MgSO4·7H2O 0.05%, FeSO4 0.001%, agar 2.0%, pH 7.0, with an osmotic stabilizer KCl 0.05% and sucrose 2%.

Single clones of stably transfected cells, isolated by limiting dilution in 96-well plates, were transferred to individual flasks and cultured in medium containing 300  μg ml−1 G418.

Infected cells were cultured in a selection medium containing 400  μg ml−1 G418 for 14 20 days and individual colonies were picked randomly and then expanded.

Individual transformants were transferred into PDA medium containing hygromycin B or Chlorimuron-ethy1and incubated until conidiogenesis.

Bone marrow was collected from both femurs of individual mice by flushing with RPMI-1640 medium containing 1% FCS.