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In the studies reported here, A. niger conidia were produced and germinated in media containing nitrate.
Due to the inability of the Δ areA mutant to utilise various nitrogen sources, no additional resistance marker was required and transformants were regenerated on media containing nitrate as the sole nitrogen source.
Abiotic stress was induced through nitrogen limitation by centrifugation, removal of media containing nitrate, and suspension of the algal pellet in sterile nitrogen-free BBM at an approximate concentration of 5 million cells per milliliter [ 45].
Although the samples are not tightly grouped, there is clear segregation of the samples of pnit-6_1.5 grown on VM-Gln (samples 1 6) and grown on media containing nitrate (samples 7 12).
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We were not surprised that use of Gln as a nitrogen source significantly increases the levels of Gln and glutamate (Glu) compared with the expression on media containing sodium nitrate.
Anabaena cells harboring a PhetR::gfp reporter gene were grown in BG-11 liquid medium containing nitrate with aeration with air (normal CO2), followed by four washes with BG-110 media lacking combined nitrogen.
He said security officials have temporarily seized 64,000 tonnes of fertilisers containing nitrate from retailers.
Turkey is temporarily suspending the sale of fertilisers containing nitrate, which can be used to make explosives, after two car bombings killed 17 people.
Solutions containing nitrate or chloride salts are examined.
Although the genes encoding nitrite dissimilation activities have been shown to be strongly induced only under anaerobic conditions, a basal level of activity could be sufficient to remove the small amount of nitrite produced by nitrate reductase in niiAΔ strains growing on media containing trace amounts of nitrate (or nitrite).
1 2 µl of the bacterial suspension was spotted onto a 1.5% wt/vol agarose growth pads (prepared using Vogel Bonner minimal media containing 0.2% wt/vol sodium nitrate and 0.01% wt/vol casamino acids) and sealed.
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