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A classical way to obtain three-fluid retention curves in porous media from measured two-fluid retention curves is based on the Leverett concept, which states that the total volumetric liquid content in a water-wet porous medium, containing water, a nonaqueous-phase liquid (NAPL) and air, is a function of the capillary pressure across the interface between the continuous NAPL and air.
Control cultures were treated with Nb-N1 medium containing water and DMSO vehicle.
Drosophila medium containing water, glucose, agar, corn meal, and the antifungal agent methyl-p-hydroxybenzoate was cooked and dispensed into glass culture vials (10 cm in height and 3.5 cm in diameter) via a self-made dispenser.
We initially maintained all flies at 23°C on a 14 10 light: dark cycle and cultured them on 8 ml of standard Drosophila porridge medium containing water, oats, sugar, brewer's yeast, and agar, plus a dilute solution of nipagin (2 g/litre of porridge medium, dissolved in 10 ml of ethanol) and propionic acid to inhibit mould and bacteria growth.
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After lentiviral infection and selection the culture medium was removed and replaced with serum free medium containing either water or 25 µM Choloroquine (CQ) for 4 hours.
7, 8 Without any effect on cell viability, the number of migrating Langerhans cells cultured in a medium reconstituted with LRP-TSW was significantly lower than in a medium containing demineralized water (relative migration index = number of migrating Langerhans cells in medium with LRPTSW/number of migrating Langerhans cells in control medium = 0.5 ± 0.3 [mean of eight donors]).
Among them, HTP is a handy and eco-friendly technology for the pretreatment of lignocelluloses as compared to others, since the medium only contains water, avoiding corrosion of equipment [ 10].
Medium samples were transferred into an optically clear 96-well plate and reaction mix (containing water soluble tetrazolium-1) was added to each well.
The content is a two-phase medium containing heavy hydrocarbons and water.
The strain was grown on a medium containing (g/l of tap water): peptone 5.0; yeast extract 3.0; KH2PO4 0.2; glucose 5.0; pH 7.2.
For live-imaging or injection assays, larvae were anesthetized in egg water medium containing 0.02% buffered Tricaine (3-aminobenzoic acid ethyl ester; Sigma-Aldrich, St Louis, MO, USA).
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