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Half-volume medium exchange was performed every 3 days with medium containing fresh cytokines.
The medium containing fresh iCR and steroids was replaced every 2 days.
To test this hypothesis, we mixed a portion of minimum medium containing various amounts of liver extract with the same volume of the medium containing fresh bacterial cells and monitored the expression of exsCEBA.
RNAi treatment was performed by incubating 1×106 cells with a mixture of 10 µg each of the two dsRNA probes for up to 8 days with changes of medium containing fresh dsRNA at days 3 and 5. Cells were harvested at different time points and processed for western blot, immunofluorescence staining or FACS analysis.
Medium containing fresh fenretinide were provided every 24 hours.
Medium containing fresh doxycycline was replenished every 2 days.
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The first column of the microtiter plate was left as a blank, containing fresh medium only.
For the growth trait measurements, the clone libraries were replicated on spectrophotometer plates containing fresh medium.
Drosophila were transferred to new chambers containing fresh medium every 2 3 days, and the number living.
Non-adherent cells in culture were transferred into new flasks containing fresh culture medium every week.
Lower chambers contained fresh medium containing 10% fetal bovine serum serving as chemoattractant.
More suggestions(15)
medium containing heavy
medium containing extracellular
medium containing nocodazole
medium containing free
medium containing exogenous
medium containing recombinant
medium containing fumarate
medium containing appropriate
medium containing additional
medium containing different
medium containing indicated
medium containing pyruvate
medium containing serial
medium containing normal
medium containing neomycin
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