In an acidic medium containing sulfuric acid ranging from 0.15 to 0.7%% (w/ w), on the contrary, it has been reported that further degradation of starch to HMF occurs above 150 °C within a few minutes [ 15].
Pre-cultures were used to inoculate 25 ml YNB medium containing acetic acid (0, 6 or 12 g l-1), at an initial OD (620nm) of 0.2.
As a characteristic feature of neural progenitor cells, these cells were able to efficiently differentiate into MAP2 and TUJ1-positive neurons when exposed to a differentiation medium containing ascorbic acid, dbcAMP, BDNF and GDNF for 14 days (Fig. 1K).
In brief, medium was exchanged 24 hours after seeding for fresh medium containing retinoc acid.
Then, the inocula were removed and fresh medium, containing phosphonoacetic acid (PAA), was added.
Following incubation for two days, the EBs were transferred, in suspension, to poly-HEMA coated tissue culture dishes to prevent cell attachment and grown in medium containing ascorbic acid [17] which was replenished every 3 days thereafter.
The ability of the pA1-A8 plasmids to support growth of G402 was assessed by challenging transformants on medium containing 5-fluoro-orotic 5-fluoro-orotic 5-fluoro-oroticacidnst plasmid pRDW10.
For the latter, osteogenic medium containing ascorbic acid (50 µg/mL) and β-glycerophosphate (10 mM) was initiated at confluence and alizarin red staining was performed at day 20.
In order to verify the status of ElpS as a lipoprotein, CB15N, CB15N elpS::elpS-3flag, CB15N ΔgspC-N elpS::elpS-3flag and CB15N ΔelpS strains were grown in poor medium containing 3H-palmitic acid.
