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The content is a two-phase medium containing heavy hydrocarbons and water.
Briefly, ILK−/− fibroblasts (Sakai et al., 2003) expressing ILK-FLAG were cultured in medium containing heavy lysine isotope for five cell doublings.
In stable isotope labeling by amino acids in cell culture (SILAC), 64 proteins are metabolically labeled by cultivating cells in culture medium containing heavy isotope versions of amino acids.
We could show that after 11 cell division cycles in medium containing heavy isotope labeled Arg/Lys the mean ratio of heavy over light isotopes in the detected peptides changed more than 20 fold from 0.51 to 11.4 indicating that the majority of proteins had incorporated the heavy isotope amino acids (Additional file 1: Figure S2A).
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The aim of this study was to evaluate the effect of welding on the corrosion resistance of the heat-affected zone (HAZ) of the AISI 444 ferritic stainless steel in medium containing Venezuelan heavy oil.
One-milliliter liquor from each reactor was used to inoculate liquid Luria Bertani (LB) medium containing the investigated heavy metal (sterilized by filtration) in that reactor.
Cells in normal medium (light culture) were treated with bortezomib, and cells grown in medium containing stable isotopes (heavy culture) were treated with vehicle.
This technique is used to quantify relative protein amounts as measured by mass spectrometry in extracts from cells grown in unlabeled (light) medium and cells grown in (heavy) medium containing non-radioactively-labeled amino acids.
Both yeast strains were grown on "regular" and "heavy" medium, containing N labeled ammoniumsulphate and N labeled amino acid supplements (Sigma Isotech).
SILAC requires cells to be grown in medium containing either light or heavy amino acids so that proteins coming from different cultures can be distinguished by the differences in their mass.
The 6-10B cells were grown in Dulbecco's modified Eagle medium containing "light" (C6) or "heavy" (C6) lysine supplemented with dialyzed fetal bovine serum at 37°C for 2 weeks.
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