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Firstly, a reasonable AI operating at Ku band was manually designed using a 130 nm technology.
The second one is an NoC router manually designed using VHDL and synthesized Altera Quartus II CAD tool.
Each model was manually designed using a parsimonious approach.
Specific primers were manually designed using Gene Runner software (Hastings Software, Inc .. Sequences of primers and PCR conditions used are shown in Table 2.> -wrap-foot> sensense; AS, antisense; Ta, annealing temperature.
The primers were manually designed using the following parameters: 19 23 bp length, 30 70% GC content, a short amplicon size (66 110 bp), and at least one primer of each pair flanking an intron-exon boundary to prevent genomic amplification (Table 1).
Primer/probe sets and the Taqman Master Mix were purchased from Applied Biosystems (Master Mix: 4364340; Hprt1: Mm01318747_g1; Jag1: Mm01270190_m1; Calb2: Mm00801461_m1; Grxcr1: Mm01217856_m1; Hsd17b7: Mm00501703_m1. Primer/probe sets for Chrna1, Chrna9 and Otof were manually designed using a tool supplied by Applied Biosystems).
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Then, the subsystems with a desired behaviour that can (resp. cannot) be easily specified manually are designed using a model checking based approach (resp. our approach).
Adequate regions were selected manually (figure 1A) and degenerate primers were designed using the Primo online software [ 17].
All primers (Table 1) were designed using the Primer 3 program (http://frodo.wi.mit.edu/primer3/) and manually checked.
All qPCR primers for the candidate transcripts were designed using Primer Express® 3.0 (ABI), the majority of which were automatically generated, the remaining were designed manually using the same software.
Previous studies focus on a case-by-case approach, where a specific event was first chosen and filtering criteria (e.g., keywords, spatiotemporal information) are manually designed and used to retrieve relevant data for disaster analysis.
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