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All non-clpP1 exon DNA sequences were manually aligned, using the principles of Oxelman et al. [33], in the sequence alignment editor Se-Al version 2.0a11 (http://evolve.zoo.ox.ac.uk/).ac.uk/
Sequences were edited and manually aligned using BioEdit v.7.0.5 (Hall 1999).
Sequences were edited and assembled in SeqMan 4.0 (DNAStar Inc., Madison, USA) and manually aligned using PAUP* 4.0b10 [56].
Sequences of env gp120 were manually aligned using ClustalW included in the software package BioEdit [55] (BioEdit v 7.0.5, Tom Hall, Ibis Therapeutics, Carlsbad, CA).
Contiguous sequences were assembled from bi-directional reads and manually aligned using Sequencher 4.5 (Gene Codes Corporation, MI) and MacClade v 4.06 [38].
The sequences were aligned with previously published OPV sequences from GenBank using the ClustalW method, and were manually aligned using MEGA software version 3.1 (Arizona State University, Phoenix, AZ, USA).
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Sequences were assembled manually and aligned using NCBI Blast and Vector NTI.
The results of Sanger sequencing were removed vector sequences manually and aligned using Blastn and MegAlign in Lasergene packages.
The virtual specimen of DH3 was mirrored in GeoMagic Studio, and manually registered (aligned) using common points along the frontal crest and sagittal suture.
Sequences were aligned manually and also aligned using Clustal W [ 92] with default parameters.
After pre-processing, the data were manually assembled and aligned using CODONCODE CodonCode Corporationn, Dedham, MA).
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