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Denoised 3-D reconstructions were manually segmented using the Amira software package (Mercury Computer System, Inc). on a Wacom Cintiq 21UX display.
Wav files representing 25 renditions of each of the selected syllables at 75d in both conditions were manually segmented using amplitude and entropy thresholds in the sound analysis window of the similarity features tab of SAP.
The hippocampi were manually segmented using the software ITK-SNAP version 3.2.0 (46).
Bilateral hippocampi were manually segmented using MEDx software and subsequently corrected for total intracranial volume.
Hippocampi were manually segmented using standard landmarks with high test-retest reliability [ 23].
One hundred individual cells from each treatment were manually segmented using the function of markup analysis program, followed by the cell measurements.
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Fluorescent coloured images were separated into individual colours and converted to binary images, manually thresholded and segmented using ImageJ (v1.41 http://rsb.info.nih.gov/ij/).
The airway was segmented using thresholding-based segmentation manually corrected slice by slice.
Total specimen area was manually segmented, and the blue-stained pixels (representing collagen) were segmented using manual intensity thresholding.
During the training stage, first, manually chosen representative images are segmented using hierarchical level set region detection.
The DICE similarity co-efficient [98] was calculated to assess the degree of overlap between manually defined ventricles and ventricles segmented using the VBM routine where: DICE = 2 ( ROI ventricles and VBM ventricles)/ ROI ventricles or VBM ventricles).
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