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Sequences for each marker were compiled into contigs and manually edited using SeqManPro version 8.0.2.
Analysis regions were defined using the templatized VOI database and were then manually edited using the fused CT and OSEM PET images as a guide.
Where necessary, the alignments were manually edited using SEAVIEW [33].
All sequences were manually edited using the program Sequencher (ver. 4.1.2, Genecodes, Madison, WI).
Sequences obtained were assembled and manually edited using SeqMan (DNAStar, Madison, WI, USA).
Sequences were aligned using Clustal X, and the alignment manually edited using the ED program of the MUST package [53].
Similar(9)
The consensus genome, consisting of an artificially assembled sequence, was annotated manually and edited using a commercial genome sequence editing software, in silico molecular cloning (in silico Biology Co., Kanagawa, Japan).
Resulting multiple protein alignments were manually verified and edited using Se-Al 2.0 (http://tree.bio.ed.ac.uk/software/seal/).ac.uk/software/seal/
The final alignments were manually inspected and edited using the sequence alignment editor SE-Al version 1.d1 software [43].
The 2506 beta-satellite monomers were aligned by CLUSTALW 2.0 [22] and manually examined and edited using MEGA 4.0 [62].
Sequences were manually aligned and edited using BioEdit vs. 7.0.5.2.
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