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Alignments with ≤ 2 mismatches were manually inspected using IGV v2.3.0 to determine if the reads cover breakpoint of each mPing subtype in each accession.
Chromatograms were manually inspected using FinchTV (Geospiza).
All alignments were manually inspected using the Se-Al v2.0a11 software [52] and analyzed individually or as concatenated files.
The remaining 8 transcripts identified by the tiling array were manually inspected using a web-based genome browser [19].
Individual chromatograms were manually inspected using FinchTV.
Hereafter, all calls were manually inspected using Chromas 2.11.
Similar(28)
Resequencing data for the same amplicons from different pups were automatically processed by PolyPhred [ 22] as well as semi-manually inspected using in-house developed software.
Predicted SNPs within the PZ, PMP gene cluster and the 77 kbp hypervariable region as well as within the six potential pseudogenes were manually and visually inspected using the BAM files generated from the BWA read mapping in Artemis.
These were visually inspected using SeqMan (DNASTAR Lasergene) and manually joined when appropriate.
The spectra obtained were manually inspected by using PeakView 1.2 software (ABSciex).
Somatic mutations were identified by muTect 1.1.4 with the default options [ 10], and manually inspected by using Integrative Genome Viewer (IGV) [ 11].
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