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Exact(7)
Two healthy rhesus macaques were used as controls.
PBL collected from macaques were used to extract RNA using the TRIzol-based (Invitrogen) isolation method and the cDNA was synthesized using the First Strand cDNA Synthesis Kit (Clontech Lab).
The following anti-human monoclonal antibodies that cross-react with rhesus macaques were used: Phycoerythrin (PE -conjugated anti-MIPE -conjugatedne D21-1351), Fluorescein isothiocyanti-MIP-1β/CCL4jugated anti-cloneclone SP34), Peridinin Chlorophyll (PerCP)-conjugated anti-CD8 (clone SK1), and Allophycocyanin (APC)-conjugated anti-CD4 (clone SK3).
In our previous study [13], Chinese-origin rhesus macaques were used as an animal model to evaluate the protective efficacy of subunit vaccines SV1 (20 µg F1 and 10 µg rV270) and SV2 (200 µg F1+100 µg rV270), and a live attenuated vaccine EV76 against subcutaneous challenge with a virulent Y. pestis 141 strain.
Three sham-operated macaques were used as controls.
The genomics extracted from the 10 Chinese and 10 Indian rhesus macaques were used as templates for the PCR amplifications.
Similar(53)
Vaxfectin adjuvanted SIVmac251 gag and env DNA immunization of rhesus macaques was used to examine magnitude, durability, and efficacy of humoral immunity.
Additionally, with macaques being used extensively as models for a plethora of human health studies besides AIDS, including SARS, tuberculosis, biodefense, transplant and cancer research; 2-MHC-haplotype-matched MCM offer enormous opportunities to rapidly advance our understanding of protective immunity.
SHIV-infected macaques being used to model HIV are precisely those in whom detection of mycobacterial infection is most critical.
Both Chinese and Indian-origin rhesus macaques are used in biomedical research, but Indian-origin animals are more prevalent.
The SIV virus isolated from macaque monkeys (SIVmac), which evolved from SIVsm in captive macaques, was used as a simian AIDS model system in Rh [ 12, 13].
Related(20)
macaques were supplied
macaques were inoculated
macaques were assessed
macaques were grouped
macaques were left
macaques were prepared
macaques were housed
macaques were divided
macaques were performed
macaques were challenged
macaques were euthanized
macaques were acquired
macaques were stimulated
macaques were observed
macaques were fasted
macaques were scored
macaques were infected
macaques were elevated
macaques was used
macaques were subjected
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