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Monocytes isolated from Pig-tailed macaques were stimulated for 8 h with TPA to enhance attachment and growth of cells.
PBL from M. tuberculosis-infected Mamu-DRB*W201+ macaques were stimulated with P65 in CFSE-based proliferation culture as we previously described [26].
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Macaque lymphocytes were stimulated with beads coated with CD3 and CD28 antibody and challenged at an MOI of 0.05 in triplicate.
To determine whether the increased expression of LTB4R mRNA in human PBMCs resulted in functional expression of LTB4R/BLT1, human and rhesus macaque PBMCs were stimulated with different concentrations of LTB4 and intracellular calcium flux was measured (as described in Methods).
Strikingly, in contrast to human mo-DCs, no IL-12p70 production was observed when macaque mo-DCs were stimulated with TLR ligands.
To assess the T-cell immune response, rhesus macaque PBMCs derived at different times following rRBD immunisation were stimulated with overlapping synthetic peptides of RBD; ELISpot analyses were then conducted.
Kids will be stimulated.
Intellectually, I am stimulated".
These are stimulated by vaccine.
"Kids are not being stimulated.
"They want to be stimulated".
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CEO of Professional Science Editing for Scientists @ prosciediting.com