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The macaques were fasted overnight before each study.
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First, twenty-four rhesus macaques were infected with the Lyme bacteria in the laboratory.
There, people and macaques were mingling on the beach in some friendly interspecies contact.
The macaques were prepared as previously described [14, 15].
Ten Rhesus macaques were infected with the SIV via intravenous injection and ten macaques were infected via a mucosal route of infection, mimicking sexual transmission.
Rhesus macaques were housed in a biosafety level 4 containment facility accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International.
Macaques were euthanized at 4 (6 macaques), 7 (11 macaques), 10 (12 macaques), 14 (6 macaques), 21 (12 macaques) and 42 (9 macaques) days p.i. in accordance with federal guidelines and institutional policies.
All macaques were positive for SIV.
Two healthy rhesus macaques were used as controls.
Nine adult, male Indian rhesus macaques were housed at CDC.
Thirteen healthy, uninfected rhesus macaques were selected for this study.
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