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Macaques were scored for clinical signs of disease prior to, and while under anesthesia.
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For long-tailed macaques, which have only single-mount copulations, copulations were scored if they involved three or more thrusts.
The frequency of 40 behaviors typical to bonnet macaques [13] was scored for each animal at 30-second intervals per session.
Surviving macaques were active, eating well, had scabbed lesions and had greatly reduced clinical scores by day 20.
First, twenty-four rhesus macaques were infected with the Lyme bacteria in the laboratory.
There, people and macaques were mingling on the beach in some friendly interspecies contact.
An additional three macaques were gestation day (GD) matched control placebo-inoculated animals.
The macaques were prepared as previously described [14, 15].
The macaques were fasted overnight before each study.
Macaques were boosted with rhFLSC protein.
Twelve macaques were vaccinated with a bacteriophage Qß-based vaccine targeting macaque CCR5 (Qß.CCR5).
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