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In vivo radiation exposure and dosimetry of Rhesus macaques were performed similar to that previous described [32].
Procedures that required BL3 animal work involving macaques were performed through the Centers for Disease Control and Prevention; procedures that required BL3 animal work involving mice were performed through the University of New Mexico Health Science Center, Albuquerque, NM.
All procedures involving rhesus macaques were performed at the Institute of Neuropathology, University Medical Center Hamburg-Eppendorf (Hamburg, Germany), in accordance with the German Animal Welfare Act and the Council Directive 86/609/EEC (Permit 33.42502/08 08.02 LAVES, Lower Saxony, Germany).
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To further correlate early host immune responses with immune activation and viremia, a comprehensive quantitative analysis of lymphokines produced in response to SHIVsf162P3 and SIVmac251-infection in macaques was performed.
Enzyme-linked immunosorbent assays (ELISA) to detect antibodies to CeHV-1 in macaque sera were performed at the B-Virus Reference Laboratory at Georgia State University (12).
Neutralization assays using rhesus macaque monoclonal antibodies were performed on viruses engineered to express single or multiple amino acid mutations.
Analyses were performed with macaque tissues.
It should be mentioned however, that previous studies on protective alleles were performed on Indian rhesus macaques and such alleles specific to Chinese macaques are yet to be defined.
Experiments were performed to clone the rhesus macaque orthologues of the relaxin-3 peptide hormone and its cognitive receptors (RXFP1 and RXFP4).
The monkey samples were from rhesus macaques used in our previous studies on SIV pathogenesis that were performed under IACUC approval from The Scripps Research Institute.
Hybridizations were performed using the RNA sampled from a 3-year-old macaque cerebrum, cerebellum, liver, and testis with duplications.
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