Sentence examples for blocking buffer and washed from inspiring English sources

Then the membrane was incubated in a goat anti-rabbit HRP-conjugated secondary antibody (1∶2,000), diluted in blocking buffer, and washed four times with 0.5% PBST.

aureus antibody in blocking buffer, washed three times with blocking buffer, incubated for 20 minutes with 40 µg/ml Texas-RedX-conjugated goat anti-mouse IgG (Life Technologies) in blocking buffer, and washed twice with blocking buffer.

Following staining, each slice was washed 3 X in 1 X PBS pH 7.4, 1% Triton X-100 at room temperature, incubated for 30 min in blocking buffer, and washed a final 3X in 1X PBS pH 7.4, 1% Triton X-100.

Sections were incubated with goat AP-anti-biotin tertiary (1 100, Vector) in blocking buffer and washed.

Then, cells were incubated 1 h at room temperature with primary antibody anti-LC3 (PMBL6-1:500; MBL, Nanterre, France) diluted in blocking buffer and washed three times before the addition of secondary antibody (Alexa Fluor 488 goat anti-rabbit, A-11034-1 500; A-11034-1 500

After adding 100  μl of blocking buffer and washing the wells with PBST buffer, a condition medium of CCN2 transfectants collected for 48 h was added to separate wells.

Primary and secondary antibody incubation was carried out in blocking buffer and washes were performed using KCM+T.

All the solutions including cell lysis buffer, blocking buffer and wash buffer were from this kit and the experiment was performed following the manufacturer's instructions.

After 1 h blocking in PBS Tween-20 (PBST) supplemented with 5% fat-free milk powder the membranes were incubated overnight with primary antibodies diluted 1 1000 in blocking buffer and subsequently washed in PBST, incubated for 1 h with secondary antibodies diluted 1 2000 in blocking buffer, and finally washed in PBST (antibodies are listed in table S1).

The membrane was washed with phosphate-buffered saline-Tween 20 (TPBS), blocked in a solution of TPBS containing 5%% nonfat dry milk (blocking buffer), and then washed three times.

samples were incubated for 1 h with goat anti-rabbit IgG conjugated with 10 nm colloidal gold particles (Sigma-Aldrich) at 1 : 50 dilution in blocking buffer, and then washed with blocking buffer (3 × 2 min ., PBS (3 × 2 min).