Sentence examples for blocking solution and washed from inspiring English sources

Sections were incubated overnight at 4°C with gentle agitation in primary antibodies diluted in blocking solution, and washed the following morning.

The cells were blocked for 30 min with 3 % BSA blocking solution and washed three times with 1 × PBS.

Then cells were incubated with anti-α-tublin antibody overnight at 4°C in blocking solution and washed with PBST for three times.

The membrane was incubated for 2 min in 100 ml of maleic acid buffer, 30 min in 1x blocking solution (Roche, USA), 30 min in 20 ml of Anti-Digoxigenin-AP Conjugate dissolved in 1x blocking solution and washed with washing buffer (Roche, USA).

After incubation with primary antibodies, the cells were washed 3 times with PBS, incubated with secondary antibodies diluted 1 1000 (Alexa fluor 594 goat anti rabbit IgG, Invitrogen, A11012; Alexa flour 488 donkey anti-mouse IgG, Invitrogen, A21202) and Hoechst, 20 μg mL 1 (Sigma, 33342) in blocking solution, and washed again 3 times with PBS.

Saponin 0.1% PBS solution was used for permeabilisation, blocking solutions and washing.

Next, cells were incubated with the 1st antibody for 1 hour at RT in blocking solution and afterwards washed five times 5 minutes with 0.02% Triton-X100/PBS.

Samples were then washed with blocking solution and mounted onto microscope slides for imaging.

The membranes were washed in blocking solution and incubated with horseradish peroxidase-coupled secondary antibody.

Grids were washed in blocking solution and incubated in goat anti-chicken or anti- mouse IgG antiserum conjugated to 6 nm gold beads (Aurion, Costerweg 5, The Netherlands).

Cells were then washed in blocking solution and incubated with Cy3 goat anti-mouse IgG (1∶300; Sigma, Dorset, UK) or FITC-conjugated goat anti-rabbit IgG (1∶200; Stratech, Newmarket, UK) for one hour at room temperature, followed by three washes with blocking solution.