Sentence examples for blocking buffer and were from inspiring English sources
The primary antibodies were diluted in the same blocking buffer and were added to detect the target proteins.
Membranes were washed three times with blocking buffer, and were incubated with alkaline phosphatase-conjugated secondary antibodies for 30 minutes.
After transfer, the membranes were saturated with blocking buffer and were incubated with rabbit immunoaffinity purified IgG anti-citrulline (Upstate Biotechnology, Lake Placid, NY, USA).
A volume of 45 μL of pre-cleared transgenic mouse brain homogenates were diluted into 450 μL of blocking buffer and were neutralized with 5 μL of 0.5 M Tris pH 6.8.
Primary antibody was diluted in blocking buffer and was added as follows: HSP27, 1 200; HSP60, 1 300; and Prx-2, 1 200.
Phosphorylated serine 10 (phospho ser10) histone H3 was detected using a similar method, except blocking buffer contained 1.5% goat serum (Santa Cruz Biotechnology, Santa Cruz, CA), 1% bovine serum albumin, and primary antibody (catalog no. 06-570; Upstate CeLake Placidg SolutioNY, Lake Placidiluteddiluted 1 500 in blocking buffer and was incubated on slides for 1 hr.
Secondary antibodies were goat anti-rabbit or goat anti-mouse IgG conjugated to AlexaFluor 488 (Invitrogen, Carlsbad, CA) (1∶500) and incubations were for 30 min. All antibodies were diluted in blocking buffer and wells were washed five times with PBS after each incubation.
Primary antibodies were diluted in blocking buffer and cells were incubated for 1 hr at room temperature.
Primary antibodies were diluted in blocking buffer and slides were incubated for 20 minutes at 37°C, followed by overnight incubation at 4°C extended by 20 minutes at 37°C.
