Sentence examples for blocking buffer and applied from inspiring English sources

Secondary antibodies were diluted in blocking buffer and applied to membranes for 1 h at room temperature.

Primary antibodies were diluted 1 2000 (MHCslow, MHC2A, calpain1, calpain2) or 1 1,000 (145-kDa cleavage product, MAFbx, MuRF1) or 1 5000 (GAPDH) in blocking buffer and applied to the membranes overnight at 4 °C.

Biotinylated goat anti-rabbit (VectorLabs, Burlingame, CA) was diluted 1∶400 in the blocking buffer and applied for 1 h at 25°C.

Secondary antibodies of goat anti-mouse Alexa-conjugated, goanti-rabbitbit Alexa-conjugated (Molecular Probes) were diluted at 1∶500 in blocking buffer and applied to cells for 1 hour at room temperature.

The EDTA plasma samples were diluted 10 times in the blocking buffer and applied to the plates.

Primary antibodies were diluted in blocking buffer and applied for 1 hr at room temperature or overnight at 4°C.

After blocking (2% bovine serum albumin, 10% normal goat serum in 0.1% Triton X-100/PBS) for 1 hour at room temperature, primary antibodies were applied in blocking buffer and incubated overnight at 4°C.

Primary antibodies were applied in blocking buffer and incubated at room temperature overnight.

Two tissue sections were incubated with the first (unlabeled) antibody (anti-carp VTG, raised in rabbit), and a solution of blocking buffer and 0.03% hydrogen peroxide was applied to the third section on each slide to demonstrate any nonspecific binding of antibody.

The secondary antibody (rabbit Alexa 568 anti-mouse IgG, Invitrogen) was applied at 1 1000 dilution in blocking buffer and incubated O/N at 4°C in the dark.

Primary antibody (Abcam ab1997 for ADAM10; Invitrogen 02-6102 for isotype) was applied to the section at 5 μg/ml in blocking buffer, and incubated for 1 hr at RT.