Sentence examples for blocking buffer and developed from inspiring English sources

The membranes were blocked in 5% non-fat dry milk in 10 mM Tris HCl, pH 7.8, 150 mM NaCl, 0.1% Tween 20, and incubated overnight with the rat monoclonal 25B6 to CTIP2 diluted 1/1000 in the blocking buffer and developed using Super Signal West Pico Chemiluminescent Substrate (Thermo scientific Pierce).

The HRP-labelled antibody was incubated for 1 h in blocking buffer and developed with ECL (ThermoScientific).

The blots were then incubated with horseradish peroxidase-conjugated secondary antibodies (BD Biosciences) in blocking buffer and developed using the ECL Western Blotting Detection Reagent GE HealthcareECL Western Blotting Detection Reagent GE Healthcare

After incubation overnight at 4 °C, blots were exposed to the appropriate horse radish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology) diluted (1 : 1000) in blocking buffer and developed using the Dura Detection Kit (Perbio Sciences, Bonn, Germany).

After 3 washes in PBS with 0.1% Tween 20, the membrane was incubated for 1 h at room temperature with HRPconjugated secondary antibody in blocking buffer and developed using an enhanced chemiluminescence Western blot detection system.

aureus antibody in blocking buffer, washed three times with blocking buffer, incubated for 20 minutes with 40 µg/ml Texas-RedX-conjugated goat anti-mouse IgG (Life Technologies) in blocking buffer, and washed twice with blocking buffer.

Then, wells were treated with blocking buffer, washed and samples were serially diluted in blocking buffer and incubated 2 hours at 37°C.

The blocking buffer then was replaced with HRP-6B6C.1 antibody diluted in blocking buffer and incubated for 1 h at room temperature.

The plates were blocked with casein blocking buffer and again incubated for one hour at 37°C on an orbital table.

Cells were washed twice in blocking buffer and then incubated in 100 μl blocking buffer for 10 min with agitation.

The membranes were blocked for 2 h in blocking buffer (Odyssey blocking buffer and PBS, 1 1 with Tween 0.05% v/v) and incubated with patient sera in a dilution of 1 10 in blocking buffer for one hour.