Sentence examples for blocking buffer and added from inspiring English sources

Animal serum was diluted 1∶100 in 1% BSA blocking buffer and added to the wells in duplicate.

To label actin structures, Alexa Fluor 568 labeled phalloidin (Invitrogen, A12380) was diluted at 1∶400 in blocking buffer and added to secondary antibody solutions.

Soluble exE-selectin/Fc and Fc-only (10 µg/ml each) were dissolved in blocking buffer and added to the cell culture dishes.

After the five washes, the competitor rabbit mAbs or sCD4 were diluted in the blocking buffer and added to the plates at three-fold serial dilutions, starting at 500 µg/mL.

Binding of the primary antibodies was detected with fluorochrome-conjugated secondary antibodies, diluted 1 200 in 5% blocking buffer and added to cells for 60 min at room temperature in the dark.

About 10 µg of peripheral blood mononucleated cell protein in 25 µl of extraction buffer was mixed with 25 µl of blocking buffer and added to individual wells on a 96-well plate with gold-conjugated mAb at the bottom of each well.

Primary antibody was diluted 1 500 in blocking buffer and was added and incubated overnight at 4 °C.

Primary antibody was diluted in blocking buffer and was added as follows: HSP27, 1 200; HSP60, 1 300; and Prx-2, 1 200.

The primary antibodies were diluted in the same blocking buffer and were added to detect the target proteins.

The primary antibody was diluted 1 1000 in blocking buffer and was added to the membrane for 2 h.

Culture supernatants were serially diluted in block buffer and added for 2 4 h to allow Env gp140 capture and incubated with a 1∶1000 dilution of pooled HIV-1 positive patient sera in block buffer.