Sentence examples for blocking buffer and mounted from inspiring English sources

Samples were then washed 3 × 30 minutes in blocking buffer and mounted on slides using Vectashield® (Vector Labs, Burlingame, CA).

Cells were then washed, incubated for 60 min with Alexa dye-conjugated secondary antibodies, rinsed with blocking buffer and mounted on slides with ProLong Antifade medium (Molecular Probes).

After antibody incubation, coverslips were washed 3 4 times with blocking buffer and mounted using ProLong Gold Antifade with DAPI (Invitrogen).

Cells were then washed, incubated for 60 min with Alexa Fluor conjugate secondary antibodies, rinsed with blocking buffer and mounted on slides with DAPI containing ProLong Gold Antifade Reagent (Invitrogen).

After three washes with blocking buffer, the sections were incubated for 1 h with fluorescein isothiocyanate (FITC -labeled and Cy3-labeled secondary antibodies, washed three times with blocking buFITC -labeledted in Vectandield mounting medium (Vector Laboratories).

The coverslips were subsequently incubated for 1 h in primary antibody followed by 40 min in secondary antibody, both diluted in blocking buffer, and finally mounted with VECTASHIELD mounting medium containing DAPI (4′,6-diamidino-2-phenylindole, Vector Laboratories, Bionordika, Stockholm, Sweden).

Slides were washed in blocking buffer and in PBS, then mounted with Vectashield mounting medium (Vector Inc).

The cells were washed three times for 5 min with blocking buffer and once with PBS and mounted with Fluoromount-G™ (Southern Biotech; Birmingham, AL, USA).

Cells were washed several times with blocking buffer and once with PBS, then mounted on to microscope slides with FluoroGel mounting medium (GeneTex), before confocal microscopy as above.

After blocking as described for ICC, sections were incubated at 4°C overnight with primary antibodies diluted in blocking buffer and then subjected to secondary incubation and mounting as described for ICC.

Primary antibodies (25 μg/ml of mAb 2E9) and Alexa Fluor 488-conjugated secondary goat anti-mouse antibodies (diluted 1 in 1 000) were diluted in blocking buffer and applied successively to cells before mounting with Citifluor.