Sentence examples for blocking buffer and was from inspiring English sources

Primary antibody was diluted 1 500 in blocking buffer and was added and incubated overnight at 4 °C.

The primary antibody was diluted 1 1000 in blocking buffer and was added to the membrane for 2 h.

Primary antibody was diluted in blocking buffer and was added as follows: HSP27, 1 200; HSP60, 1 300; and Prx-2, 1 200.

Phosphorylated serine 10 (phospho ser10) histone H3 was detected using a similar method, except blocking buffer contained 1.5% goat serum (Santa Cruz Biotechnology, Santa Cruz, CA), 1% bovine serum albumin, and primary antibody (catalog no. 06-570; Upstate CeLake Placidg SolutioNY, Lake Placidiluteddiluted 1 500 in blocking buffer and was incubated on slides for 1 hr.

The primary antibodies were diluted in the same blocking buffer and were added to detect the target proteins.

Membranes were washed three times with blocking buffer, and were incubated with alkaline phosphatase-conjugated secondary antibodies for 30 minutes.

After transfer, the membranes were saturated with blocking buffer and were incubated with rabbit immunoaffinity purified IgG anti-citrulline (Upstate Biotechnology, Lake Placid, NY, USA).

A volume of 45 μL of pre-cleared transgenic mouse brain homogenates were diluted into 450 μL of blocking buffer and were neutralized with 5 μL of 0.5 M Tris pH 6.8.

Following the EdU detection reaction, larvae were incubated in blocking buffer and immunostaining was performed as described above.

Primary antibodies were diluted in blocking buffer and incubation was performed overnight at 4°C (cyclin D1 and p21 1:500 (Cell Signaling); cyclin E (sc-481) 1 100 (Santa Cruz); CDKN2A 1 200 (Thermo Scientific)).

The cell pellet was then resuspended in 300 500  μl of blocking buffer and fluorescence was read by a flow cytometer.