In such artificial applications of RNA sequence, we have to design an RNA sequence that folds into a desired structure (target structure) to realize a desired function.
That means using a guide RNA to direct a CRISPR protein to search for any specific DNA or RNA sequence.
1) It's not clear if the shRNAs actually target the novel RNA sequence that the authors identified, they target a-sat which colocalizes with the novel RNA sequence.
They also tested a completely random RNA sequence.
The RNA sequence work of Sanger's group did confirm the genetic code.
In fact, there are many groupings of bacteria that are not supported by RNA sequence analysis.
Oligonucleotide-based therapeutics are commonly used to target RNA sequence.
RNA sequence context matters in the termination of protein translation.
PUMILIO is a prototypical PUF protein whose RNA sequence specificity is simple, elegant, and predictable.
Our method creates a stable map of conformational space for a given RNA sequence.
