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Conclusion: Spontaneous secondary structure formation can influence the outcome of RNA gene amplification and should be considered an important factor when designing primers and adopting protocols for RNA gene amplification.
Specifically, Sykes's team looked at the 12S RNA gene, something that has already been analysed in all known mammalian species.
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools.
Bacterial, archaeal and eukaryotic assemblages are surveyed using small-subunit ribosomal RNA gene amplicon sequencing methods.
Quast, C. et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools.
Marques da Silva, R. et al. Bacterial diversity in aortic aneurysms determined by 16S ribosomal RNA gene analysis.
First, we amplified 16S and 18S ribosomal RNA gene sequences, which revealed only the presence of bacteria in the lagoons.
The functional composition of microbial community samples from several environments is predicted based on 16S ribosomal RNA gene sequencing data.
16S RNA gene which is currently used for bacterial species identification presents intraspecies heterogeneity.
A set of six primers was designed by targeting the 16S ribosomal RNA gene.
New primers targeting the 18S ribosomal RNA gene were designated for specific amplification.
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