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c RNA display technology.
RNA display technology includes mRNA display and ribosome display [186].
In vitro display technologies can be further classified into RNA display and DNA display technologies.
Notably, boule and vasa RNA display independent subcellular distribution.
Expression data generated from 10 pg of input RNA display a reduced number of transcripts detected (18 at log2≥7), with a 34% overlap with those detected at 500 pg input using the WT-Ovation One-Direct system (Figure 5).
Because the probability of finding functional domains in a stochastic sampling of protein sequences was anticipated to be very low, perhaps too low to detect with conventional technologies, we used a cell-free selection system called messenger RNA display to construct a starting library of greater than 1012 non-redundant random-sequence proteins [7].
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Purified virions from symptomatic leaves remain non-infectious and Northern blot analysis of virion RNA displayed packaging defects.
MT3-MMP RNA displayed one peak at stage 61, while TIMP-3 RNA levels dipped at stage 61.
Embryos injected with zfBili RNA, but not control RNA, displayed a slight expansion of anterior structures, which is consistent with diminished Wnt/β-catenin signaling (data not shown) [18].
In addition, the boule RNA displays a dynamic subcellular distribution relative to stages of oogenesis: It disperses evenly in small oocytes at stage I, concentrates in several patches in growing oocytes at stage II, and re-disperses again in oocytes from stage III onwards (Figure 4A C).
Intriguingly, a structure of ADAR2 bound to RNA displays sequence-specific contacts between the protein and the minor groove [ 29].
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