Sentence examples for rna clip from inspiring English sources

PTR: Post-transcriptional regulation; UTR: Untranslated region of mRNA; RBP: RNA-binding protein; ncRNA: Non-coding RNA; CLIP: Cross-linking and immunoprecipitation; AURA: Atlas of UTR Regulatory Activity BMFF: Boolean matrix factorization; NSPDK: Neighborhood subgraph pairwise distance kernel; AUROCC: Area under the receiver operating characteristic curve.

FUS RNA CLIP-tag distributions are similar in mouse and human neurons, indicating conserved functions for these genes, and target mRNA GO terms implicated FUS in the regulation of vital genes for neuronal maintenance, development and function [ 31, 71].

Note that FUS and EWS target genes characterized in terms of expression in this report were also identified by RNA-CLIP in Hoell et al. [ 26].

Additionally, genome-wide RNA cross-linking and IP (RNA-CLIP) analysis has shown that FUS interacts with antisense RNA originating from promoter regions [ 44].

FUS RNA-CLIP analyses showed widespread cross-linking along the whole length of associated pre-RNAs, suggesting that FUS associates with target RNAs until splicing is completed [ 44, 69– 71].

To determine the extent to which ELAV- Ubx RNA interactions take place within the physiological environment of the developing embryo we developed a series of RNA cross-linking and immunoprecipitation (RNA-CLIP) assays using embryonic nuclear extracts (Fig. 2F).

For A. and B. legend details see Additional file 16. (DOCX 693 kb) Additional file 18: Genes co-identified in the hereby presented ChIP-seq data and RNA-CLIP from Hoell et al. [ 26 ].

A comparison of RNA-CLIP identified RNA targets for FUS and EWS [ 26] and the genes hereby identified by ChIP-seq, showed that 37 out of 134, and 68 out of 241 genes for FUS and EWS, respectively, were overlapping (Additional file 18).

One recent innovation is the Cross-Linking ImmunoPrecipitation (CLIP); CLIP exploits photoreactive residues in RNA and polypeptides to generate covalently linked complexes.

Pyro-CLIP experiments generate hundreds of thousands of RNA fragments (CLIP amplicons) to which an RBP binds.

In a CLIP-pyrosequencing experiment, the resolutions of the detected protein binding regions are partially determined by the length of the detected RNA fragments (CLIP amplicons) after trimming by RNase digestion.