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These mutations were subjected to G418 at two different concentrations, 250 and 350 µg/mL, respectively (Walker et al. 2003), to screen the strains harboring stable resistance genes.
The RT-PCR data of the transcription levels of selected genes with promoter mutations were subjected to statistical analysis.
An exceptionally high incidence of tumors possessing mutations in a gene suggests that cells containing these mutations were subjected to clonal expansion in multiple individuals due to certain selective advantages provided by these mutations during multi-step carcinogenesis [26].
Fifty seven (57) IM-resistant CML patients without BCR-ABL mutations were subjected to HOXA4 methylation analysis.
The genomic DNA samples from the 5 PZA-resistant mutants that were positive for PZase and did not have pncA mutations were subjected to whole genome sequencing using Illumina HiSeq 2000 machine.
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The observations made here with Rad21+/− mice indicate that one Rad21 allelic mutation confers a level of IR sensitivity that eclipses that observed in Atm heterozygous mice and accordingly raises the issue that should cancer patients with Rad21 or potentially other cohesin mutations be subjected to RT they may suffer adversely from standard RT protocols.
Assuming synonymous mutations are subjected to almost strictly neutral selection, ω<1, ω=1, and ω>1 represent negative selection, neutral evolution, and positive Darwinian selection, respectively [ 65].
In principle, this somatic evolution should follow the same Darwinian logic as evolutionary processes in populations in nature: diverse heritable types arising as a result of mutations are subjected to selection, resulting in expansion of the fittest clones.
Control and Mnn1 null embryos and larvae heterozygous for the mwh1 mutation were subjected to repeated but short 20 min heat shock treatments at 37°C.
Women in families without a demonstrable BRCA mutation were subjected to annual mammography.
Women from breast cancer families without a demonstrable BRCA1/2 mutation were subjected to annual mammography from age 30 years onwards.
Related(20)
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