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Variants were subjected to many comparisons with external data sources.
As both bacterial variants were subjected to the host environment, they underwent additional changes in gene expression.
Following appropriate (quality) checks three pairs of protein variants were subjected to a population based association test that allows for detecting statistical interaction between the variants.
705 pmol of MgZ-5 sequence variants and 718 pmol of MgZ-7 variants were subjected to 6 rounds of selection based on the original selection scheme described above.
To determine client protein-binding affinities, the purified human PD variants were subjected to a fluorescence anisotropy-based binding assay, using the fluorescein-labeled P5 (F-P5) peptide.
Those 121 variants were subjected to validation by a direct Sanger sequencing, and 67 were confirmed to be present in the tumor, but absent from the normal samples.
The phosphorylated hMyo3A variants were subjected to SDS-PAGE, and the incorporation of P into phosphorylated kinase domain was detected by phosphorimaging using the Typhoon 9410 variable mode imager (GE Healthcare, Wauwatosa, WI).
(B ) The ER membrane targeted RNCs of XBP1u variants were subjected to a proteinase K (PK) accessibility assay in the presence of the indicated salt concentrations and salt plus puromycin (pur).. * indicates the inclusion of a detergent in the reaction.
The variant with the largest improvement in initial rate was then used as the template for the next round of error-prone PCR or several variants were subjected to DNA shuffling.
Similar(2)
Therefore, site-saturation mutagenesis was performed on both positions and a complete set of all 38 variants was subjected to rapid HPLC screening for improved ampicillin synthesis.
All variants are subjected to continuous selection; therefore, only the most fit are able to survive and spread [ 8].
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