Sentence examples for transformants were subjected from inspiring English sources
Transformants were subjected to three rounds of screening.
Selected transformants were subjected to Reverse Northern analysis.
Finally, the obtained positive transformants were subjected to single spore isolation.
After purification, the pUC-M-whip∗ transformants were subjected to counter selection.
All obtained transconjugants and transformants were subjected to typing by a PCR method described previously based on replicons of the major plasmid incompatibility groups among Enterobacteriaceae [ 20].
Transformants were subjected to Southern blot analysis, and MV3.2 was selected as this transformant contains a single copy of the pBB19-3 platmid athehe pyrG locus.
To obtain a homokaryotic ∆Smmob3:: hph strain in a wt background, a primary transformant was subjected to conventional genetic analysis.
The expression of the heterologous gluc gene was further increased in transformants containing the gluc gene under control of the heterologous tandem promoter including the heat shock promoter, if the transformants were subject to a temporary shift to higher temperatures.
Transformants verified by colony PCR were subjected to S1 nuclease pulsed field gel electrophoresis (PFGE) [ 10], PCR-based replicon typing (PBRT) (Diatheva, Fano, Italy), and antimicrobial susceptibility testing (as described above).
Whole-cell lysates of the transformants were prepared and equal amounts of protein from each strain were subjected to immunoblot analysis.
Transformant-colonies grown on high stringency selection media lacking, tryptophan, leucine and histidine (SD-T-L-H) were subjected to a colony-lift filter assay.
