Sentence examples for mutations were made from inspiring English sources

Mutations were made using PCR, and the mutated fragment was subcloned back into pCS2-Nrp1 using endogenous restriction sites.

The mutated 3'-UTR of CCND2 was synthesized after point mutations were made in several bases within the binding sites of these PCR products.

Regions where these compensatory mutations were made is marked.

Mutations were made to the ribozyme sequences so that the ribozyme's preferred secondary structure would form.

All mutations were made independently in pET28a-mphI, pET28a-ery_1, pGDP4-mphI, and pGDP4-ery_1.

(a) AA>CC substitution mutations were made at the putative FRα binding sites on Hes1 or FGFR4 promoters (mutated sites are shown in the supplemental information).

Mutations were made for each enzyme in the vicinity of the active site and we examined these variants for glycosylase and lyase activity.

Figure 2 shows a schematic representation of the evolutionary algorithm implemented, in which single gene mutations were made and then selected according to the fitness functions discussed in the Methods section.

It is worth noting, however, that even if mutations were made to the entire RBD in vivo, Tat is still capable of accessing pTEFb from small complexes of pTEFb Brd444.

To investigate a possible connection between activity at low pH and protonation of C41, mutations were made that were predicted to either eliminate protonation or alter the nature of the tertiary interaction upon protonation.

To study the role of the potential hydrogen bonds that connect the two domains, a series of mutations were made to the hydrogen bond donors and acceptors along the domain interface.