Sentence examples for mutations obtained by from inspiring English sources

As described above, r = 1,250 for loss-of-function mutations obtained by EMS mutagenesis.

Interestingly, mutations in such residues did not change the effector spectrum of HbpR and neither did the other generated mutations, whereas previous mutations obtained by directed evolution of HbpR that did change the effector spectrum from 2-HBP to 2-chlorobiphenyl mapped in completely different areas of the A-domain (Text S1).

We characterized the impact of mutations in the LOV2 domain described in the literature as well as mutations obtained by random mutagenesis.

We present here the first comparison of the sensitivity of T7E1 and Surveyor EMC assays on deletions and point mutations obtained by ZFNs in frog embryos.

Bacteriophage resolvases and single-stranded nucleases are used commonly in the assay but have not been compared side-by-side on mutations obtained by engineered nucleases.

We present the first comparison of the sensitivity of T7E1 and Surveyor EMC assays on deletions and point mutations obtained by zinc finger nuclease targeting in frog embryos.

MTHFR genotypes were identified by seeing the presence or absence of 677C→T mutation obtained by PCR followed by Hinfl restriction digestion.

In our previous study [ 24], the corresponding reciprocal L725V mutation obtained by site-directed mutagenesis of PaTPS-Iso resulted in the formation of sandaracopimaradiene as a minor product.

dsp1 1 is a null allele of dsp1, which is maintained as a homozygous strain [ 37]. corto, corto 07128 and corto L1 are loss-of-function alleles: a deficiency, a P-element insertion and an uncharacterized mutation obtained by EMS mutagenesis, respectively.

For SIM I simulations, 100 sets of random passenger mutations were obtained by shuffling the observed mutations from the TCGA ovarian dataset while preserving nucleotide context and mutation type, but ignoring gene-specific factors that affect mutation rate such as replication timing and expression level (see Fig. 2 and Section 2.3 for details of these gene-specific factors).

The variants with multiple mutations were obtained by introducing additional mutation(s) in a given variant, using the overlap PCR workflow described above.