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Mutants obtained by rational design at this key position showed significant changes in acyltransfer activity.
The mutants obtained by RGMS showed great genetic diversity that could be further exploited in inverse metabolic engineering.
Using the software tool MZmine 2, the metabolite profile of the generated mutants obtained by HPLC HRMS was compared, revealing further intermediates.
To produce a device in which the d-amino acid composition does not alter the results, both the wild-type and a number of mutants obtained by rational design and directed evolution approaches were used.
Our results show that recombining active-site mutants obtained by saturation mutagenesis can rapidly destabilise critical networks of co-evolved residues, whereas beneficial single mutants can be retained and improved upon by randomly recombining them with natural variants at other positions in the network.
In the current study, we first characterized two loss-of-function mutants obtained by screening a randomly mutated AAV4 capsid library.
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Takano, N. et al. Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams.
The effect of medium components on cordycepin production by Cordyceps militaris mutant obtained by ion beam irradiation was investigated.
A PylRS Y384F) mutant obtained by random screening exhibited higher in vitro aminoacylation and in vivo amber suppression activities with BocLys, AlocLys, and pyrrolysine than those of the wild-type PylRS.
An AtSPL8-knockout mutant obtained by transposon tagging showed disrupted trichome formation (Unte et al. 2003).
A4 is the wild-type strain that has been sequenced [23], while A91 is a spore-colour mutant obtained by ultraviolet irradiation of a different wild-type environmental isolate [61].
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